Cloning and characterization of the groE locus from Actinobacillus pleuropneumoniae.
FEMS Microbiol Lett
; 147(1): 11-6, 1997 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-9037757
ABSTRACT
A 4.4-kb DNA fragment was cloned from Actinobacillus pleuropneumoniae (strain 4074, serotype 1) by genetic complementation with Escherichia coli groES-groEL mutant strains. Sequence analysis of this fragment revealed a purine nucleoside phosphorylase (DeoD)-encoding gene homolog (deoD), heat-shock response-encoding genes for the small (groES) and large subunits (groEL) and a partial open reading frame encoding an alcohol dehydrogenase homolog (adhE). The predicted amino-acid sequence of groES and groEL genes showed extensive sequence identity (80-95%) with other Pasteurellaceae. The gene organization surrounding the groE locus was different from that of Haemophilus infuenzae. When expressed in E. coli, groES-groEL genes were capable of complementing the growth of a lambda lytic phage, indicating a structural as well as functional conservation.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Actinobacillus pleuropneumoniae
/
Proteínas de Choque Térmico
Idioma:
En
Año:
1997
Tipo del documento:
Article