Optimisation of methodology for enumeration of xerophilic yeasts from foods.
Int J Food Microbiol
; 35(2): 109-16, 1997 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-9105918
ABSTRACT
Xerophilic yeasts grow in intermediate moisture foods (aw, 0.65-0.85) such as sugar syrups, fruit concentrates, jams and brines. Non-osmophilic yeasts are enumerated by diluting in 0.1% peptone and then plated onto media such as malt extract or glucose yeast extract agar. In the presence of moulds the yeasts are enumerated in dichloran rose bengal chloramphenicol agar (DRBC). These procedures were demonstrated to be unsatisfactory for the enumeration of xerophilic yeasts in low aw foods. Investigations using pure cultures of xerophilic yeasts as well as naturally contaminated apple juice concentrates and glacé cherries have shown that a reduced aw diluent, in particular 30% w/w glycerol in combination with tryptone 10% glucose yeast extract agar (TGY) optimises the recovery of the yeasts, especially sublethally injured cells. The inclusion of sodium chloride in either the diluents or the culture media was not necessary to optimise the recovery of D. hansenii growing in 20% sodium chloride broths.
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Banco de datos:
MEDLINE
Asunto principal:
Levaduras
/
Microbiología de Alimentos
/
Micología
Idioma:
En
Año:
1997
Tipo del documento:
Article