KpnAI, a new type I restriction-modification system in Klebsiella pneumoniae.
J Mol Biol
; 271(3): 342-8, 1997 Aug 22.
Article
en En
| MEDLINE
| ID: mdl-9268663
ABSTRACT
The KpnAI restriction-modification (R-M) system has been identified in Klebsiella pneumoniae strain M5a1. The restriction gene of KpnAI was first cloned into pBR322 using an r-m+ M5a1 derivative and phage SBS for screening. Subsequently, an adjacent DNA fragment showing modification activity was cloned into pUC19. A total of 7.2 kb DNA sequencing data revealed three open reading frames, corresponding to hsdR, hsdM and hsdS genes of type I R-M systems. The predicted hsdR, hsdM and hsdS-coded peptides shared 95%, 98% and 44% identity, respectively, with the corresponding peptides of the recently identified StySBLI system, a prototype of the type ID family. This high homology suggests that KpnAI is also a member of the type ID family. The KpnAI system seems to be the first type I system identified in Klebsiella species.
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Banco de datos:
MEDLINE
Asunto principal:
Enzimas de Restricción-Modificación del ADN
/
Klebsiella pneumoniae
Idioma:
En
Año:
1997
Tipo del documento:
Article