Increases in cytosolic Ca2+ accompany basophil activation by eosinophil granule major basic protein.
Immunol Lett
; 58(1): 37-42, 1997 Jun.
Article
en En
| MEDLINE
| ID: mdl-9436467
ABSTRACT
The response of basophil cytosolic free Ca2+ concentrations ([Ca2+]i) to stimulation by eosinophil granule major basic protein (MBP) was assessed using digital video microscopy. MBP stimulated an elevation in basophil [Ca2+]i that, in general, developed slowly and increased monotonically. In some experiments, however, the average rise in [Ca2+]i was biphasic, with an initial transient increase followed by a larger and more sustained elevation. At the single cell level MBP stimulation caused frequent, asynchronous oscillations in [Ca2+]i that ranged up to 300 nM in amplitude. Chelation of extracellular Ca2+ selectively blocked the second, sustained rise in [Ca2+]i as well as MBP-induced histamine release. Within individual experiments, the increase in [Ca2+]i paralleled the level of histamine release. These results demonstrate that, unlike the activation of neutrophils or eosinophils, basophil activation by MBP correlates with an elevation in [Ca2+]i. Further, the MBP induced [Ca2+]i response exhibits several features in common with the IgE-mediated [Ca2+]i response.
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Banco de datos:
MEDLINE
Asunto principal:
Ribonucleasas
/
Basófilos
/
Proteínas Sanguíneas
/
Calcio
Límite:
Humans
Idioma:
En
Año:
1997
Tipo del documento:
Article