Cytochemical and immunocytochemical investigations on epidermal mitochondria-rich cells in Salamandra salamandra salamandra (L.) larvae.

ABSTRACT

In the present study we set out to investigate the expression of E-cadherin, N-cadherin, beta 1-integrin, fibronectin and vitronectin in the mitochondria-rich cells (MRC) of the skin of Salamandra salamandra salamandra. Moreover MRC were stained with five lectins (Triticum vulgaris; Dolichos biflorus; Glycine max; Arachis hypogaea and Canavalia ensiformis). Larval MRC expressed both adhesion molecules and extracellular matrix glycoproteins and bound all lectins tested. Juvenile MRC did not react with the antisera utilized, but they stained with the lectins. Both the lectins and the regulatory molecules proved to be good cytochemical markers for distinguishing morphologically differentiated MRC during the larval life of Salamandra salamandra salamandra. The adhesion molecules and matrix glycoproteins are of great utility for maintaining the correct tissue architecture. In Salamandra salamandra salamandra larvae these molecules may be crucial for stability and for the correct localization and fate of all skin elements, including specialized cells such as larval MRC.