A versatile method for purification of diphtheria toxoid for immunization purposes.
Arch Immunol Ther Exp (Warsz)
; 24(3): 349-58, 1976.
Article
en En
| MEDLINE
| ID: mdl-962512
ABSTRACT
Diphtheriae growth supernatant. The toxoid was quantitatively recovered from the immunosorbent in two fractions the first in 0.005 M hydochloric acid and the second in 6' M urea containing 0.1 M HCL. Both fractions were soluble in aqueous solvents, and both had a similar specific activity between 200 and 250 Lf/mg protein, and about 1.5 protective unit (ED50) per l Lf unit, as assayed in guinea pigs. Using the same procedure, the specific activity of a commerical toxoid preparation, purified and concentrated, was increrased two fold, from 130 Lf/mg to 260 Lf/mg protein.
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Banco de datos:
MEDLINE
Asunto principal:
Toxoide Diftérico
Límite:
Animals
Idioma:
En
Año:
1976
Tipo del documento:
Article