High-performance liquid chromatographic determination of total plasma homocysteine with or without internal standards.
J Chromatogr A
; 828(1-2): 397-400, 1998 Dec 18.
Article
en En
| MEDLINE
| ID: mdl-9916320
ABSTRACT
Hyperhomocysteinemia is an independent risk factor for atherosclerosis and vascular occlusive disease. Assessment of total plasma concentration of homocysteine (tHcys) requires accurate and reproducible measurements. The aim of this study was to test a rapid isocratic HPLC method for tHcys analysis with an internal standard (I.S.) of alpha-mercaptopropionylglycine (MPG), 2-mercaptoethylamine (ME), or N-acetylcysteine (NAC) or without I.S., and to verify whether the use of an I.S. improves the precision. The method without I.S. showed an excellent linearity (y = 1.59x - 0.15, r = 1), recovery (100%) and a within-assay relative standard deviation (R.S.D.) of 1.2%. Instead, in our hands, the presence of I.S.s decreased the reproducibility (within-assay R.S.D. ranged from 4.5 to 6.5%) and lengthened the chromatogram by up to four to five times. In conclusion, HPLC measurement of plasma tHcys without I.S. improves accuracy with respect to determination with I.S.; moreover, this approach allows to routinely process larger amounts of plasma samples.
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Banco de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
/
Homocisteína
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Año:
1998
Tipo del documento:
Article