Changes of platelet phosphodiesterase activity in patients with acute cerebral infarction and its influencing factors / 中华神经医学杂志

ABSTRACT

Objective To observe the changes of activities of platelet phosphodiesterase(PDE)subtypes in patients with acute cerebral infarction,and explore their influencing factors.Methods The platelet PDE activity,cyclic nucleotide level and[Ca2+]i concentration of 30 patients with acute cerebral infarction were detected on the 1st, 4th,8th and 15th d of onset.Ten healthy individuals of the same age ranges were chosen as control group.Results On the 1st,4th and 8th d of onset,as compared with those in the healthy individuals,the activities of platelet PDE2 and PDE3 and the level of cyclic adenosine monophosphate(cAMP)were obviously decreased,and the[Ca2+]i level was significantly increased in patients with acute cerebral infarction(P<0.05); while the PDE5 activity and cyclic gnanosine monophosphate(cGMP)level in patients with acute cerebral infarction did not change obviously.[Ca2+]iwas linearly correlated with cAMP(R2=0.921,P<0.05);however,the activities of PDE2 and PDE3 showed no correlation with both levels of[Ca2+]i and cAMP. Conclusion Decreased cAMP level and increased[Ca2+]i concentration are noted in patients with acute cerebral infarction,and the platelet is activated at this time points.Platelet PDE2 and PDE3 play protect roles in acute cerebral infarction through decreasing their activities,slowing the cAMP decreased level and inhibiting the activity of platelet.