Screening of toxic potential of graphene family nanomaterials using in vitro and alternative in vivo toxicity testing systems
Environmental Health and Toxicology
; : e2015007-2015.
Article
en En
| WPRIM
| ID: wpr-137586
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVES: The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nananomaterials (GFNs) in alternative in vitro and in vivo toxicity testing models. METHODS: The GFNs used in this study are graphene nanoplatelets ([GNPs]-pristine, carboxylate [COOH] and amide [NH2]) and graphene oxides (single layer [SLGO] and few layers [FLGO]). The human bronchial epithelial cells (Beas2B cells) as in vitro system and the nematode Caenorhabditis elegans as in vivo system were used to profile the toxicity response of GFNs. Cytotoxicity assays, colony formation assay for cellular toxicity and reproduction potentiality in C. elegans were used as end points to evaluate the GFNs' toxicity. RESULTS: In general, GNPs exhibited higher toxicity than GOs in Beas2B cells, and among the GNPs the order of toxicity was pristine>NH2>COOH. Although the order of toxicity of the GNPs was maintained in C. elegans reproductive toxicity, but GOs were found to be more toxic in the worms than GNPs. In both systems, SLGO exhibited profoundly greater dose dependency than FLGO. The possible reason of their differential toxicity lay in their distinctive physicochemical characteristics and agglomeration behavior in the exposure media. CONCLUSIONS: The present study revealed that the toxicity of GFNs is dependent on the graphene nanomaterial's physical forms, surface functionalizations, number of layers, dose, time of exposure and obviously, on the alternative model systems used for toxicity assessment.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Óxidos
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Reproducción
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Técnicas In Vitro
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Tamizaje Masivo
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Caenorhabditis elegans
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Pruebas de Toxicidad
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Medición de Riesgo
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Nanoestructuras
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Células Epiteliales
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Grafito
Tipo de estudio:
Diagnostic_studies
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Etiology_studies
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Prognostic_studies
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Risk_factors_studies
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Screening_studies
Límite:
Humans
Idioma:
En
Año:
2015
Tipo del documento:
Article