Effect of trihydroxybenzoic acid dimmer on induction of apoptosis of HL-60 cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 42-47, 2009.
Article
en Zh
| WPRIM
| ID: wpr-232600
Biblioteca responsable:
WPRO
ABSTRACT
Trihydroxybenzoic acid dimmer is an anti-tumor compound which is separated from water-caltrop. This study is aimed to investigate its anti-proliferation effect on HL-60 cells and the possible mechanism of inducing apoptosis. MTT assay was used to test HL-60 cells proliferation. The apoptosis, ROS levels and the mitochondrial membrane potential were detected by flow cytometry. Cytochrome c released from mitochondria to cytosol fraction was detected by Western blotting. The activity of Caspase-9 and Caspase-3 were measured by colorimetric method. It was found to inhibit HL-60 cells proliferation in a dose and time-dependent manner. Compared with control group, it caused the increase of ROS levels and a concomitant dissipation of the mitochondrial membrane potential and induced cytosolic accumulation of cytochrome c and activities Caspase-9 and Caspase-3. Therefore it can be concluded that mitochondrial-dependent pathways was involved in its induction of apoptosis of HL-60 cells.
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Farmacología
/
Fisiología
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Plantas Medicinales
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Química
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Especies Reactivas de Oxígeno
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Apoptosis
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Células HL-60
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Citocromos c
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Caspasa 3
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Caspasa 9
Límite:
Humans
Idioma:
Zh
Año:
2009
Tipo del documento:
Article