Preparation of the monoclonal antibody against human Bocavirus VP2 protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 63-65, 2012.
Article
en Zh
| WPRIM
| ID: wpr-246179
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To express and purify HBoV VP2 protein, and the monoclonal antibody against HBoV VP2 protein was prepared with hybridoma technique.</p><p><b>METHODS</b>The HBoV VP2 cloned into vector pET-30a was expressed in E. coil. After purified by immobilized metal affinity chromatography, the BALB/c mouse was immunized with purified protein as antigen. The positive hybridoma cells were screened with hybridoma technique and ELISA assay. Isotype and titer of the monoclonal antibody were detected.</p><p><b>RESULTS</b>The recombinant HBoV VP2 protein was expressed and purified, and then the monoclonal antibody was obtained with hybridoma technique. The titer of the IgG monoclonal antibody was up to 14 x 10(5).</p><p><b>CONCLUSION</b>Monoclonal antibody against recombinant HBoV VP2 protein was prepared and the antibody titer was high. This work may provide a new method in rapid diagnosis and study of HBoV.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Plásmidos
/
Proteínas Recombinantes
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Proteínas de la Cápside
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Alergia e Inmunología
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Bocavirus Humano
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Genética
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Hibridomas
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Ratones Endogámicos BALB C
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Anticuerpos Monoclonales
Límite:
Animals
Idioma:
Zh
Año:
2012
Tipo del documento:
Article