Research on phytoestrogenic effect of formononetin / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>Research on the phytoestrogenic effect and its possible mechanism of formononetin.</p><p><b>METHOD</b>To evaluate the estrogenic effect and mechanisms of formononetin through the test of its influence on proliferation and ER subtype expression of T47D cells.</p><p><b>RESULT</b>The proliferation rates of T47D cells treated with 1 x 10(-7) -1 x 10(-6) mol x L(-1) formononetin were not increased. On the influence of ICI182, 780, the proliferation rates of T47D cells treated with 1 x 10(-7) 1 x 10(-6) mol x L(-1) formononetin were decreased. Formonenetin could induce the augment of ERalpha expression significantly of T47D.</p><p><b>CONCLUSION</b>Formonenetin has phytoestrogenic effect Formonenetin can not accelerate ER(+) T47D cell proliferation. But the expression level of ERalpha subtype in T47D cells change significantly with certain concentrations of formonenetin.</p>