Study on alternative methods for the acute oral toxicity in detection of chemicals / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 431-434, 2011.
Article
en Zh
| WPRIM
| ID: wpr-272574
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate three alternative methods for LD50 test-Fixed Dose Procedure (FDP), the Acute Toxic Class Method (ATC) and Up and Down Procedure (UDP).</p><p><b>METHODS</b>Female SD rats (8-12 weeks of age, 160-200 g) were used. Three alternative methods from OECD were applied to assess 22 chemicals (10 cosmetic raw materials and 12 raw materials of personal and home care products). The toxicity ranking for tested chemicals was established according to Globally Harmonized System (GSH). The results LD50 test were compared for the consistency and correlation between alternative methods and traditional test.</p><p><b>RESULTS</b>For cosmetic raw materials, the concordance rate of the three alternative methods was 80% (8/10); for raw material of personal and home care products, the concordance rates of FDP, ATC and UDP was 91.7% (11/12), 75.0% (9/12) and 83.0% (10/12), respectively. The number of animals required in three alternative methods was significantly lower than that in traditional test (P < 0.05), but the time required in three alternative methods was significantly higher than that in traditional test (P < 0.05).</p><p><b>CONCLUSIONS</b>High consistency and correlation were found between each alternative method and LD50 test. FDP may be more potential when applied to assess acute oral toxicity of cosmetic raw materials.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Sustancias Peligrosas
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Administración Oral
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Ratas Sprague-Dawley
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Cosméticos
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Pruebas de Toxicidad Aguda
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Relación Dosis-Respuesta a Droga
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Toxicidad
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Dosificación Letal Mediana
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Métodos
Tipo de estudio:
Diagnostic_studies
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Risk_factors_studies
Límite:
Animals
Idioma:
Zh
Año:
2011
Tipo del documento:
Article