Establishment of primary cultured neurons model of mouse embryo with HSV-1 infection / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 237-239, 2003.
Article
en Zh
| WPRIM
| ID: wpr-281769
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To establish a neurons model for herpes simplex virus type 1 (HSV-1) infection using cultures of mouse embryo.</p><p><b>METHODS</b>The cortical neuron of 14-16 day old mouse embryo were cultured and infected with HSV-1. Microscopic examination, immunofluorescent test, MTT colorimetric analysis and flowcytometric assay were used to detect the immunofluorescent reaction of normal and infected neurons and astrocytes, respectively. The percentage of the two kinds of cells were measured.</p><p><b>RESULTS</b>The mouse neurons could proliferate well in vitro. The neurons amount met the requirement of cytological experiment after treatment with Ara-C in the tissue culture. HSV-1 could infect the neurons directly, the activity of the infected neurons was obviously reduced.</p><p><b>CONCLUSION</b>HSV-1 could successfully infect the primary cultured neurons. The model may provide a useful approach for studying the pathogenesis of herpes simplex virus encephalitis.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Fisiología
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Virología
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Células Cultivadas
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Herpesvirus Humano 1
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Técnicas de Cultivo de Célula
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Embrión de Mamíferos
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Herpes Simple
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Métodos
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Neuronas
Límite:
Animals
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Female
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Humans
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Male
Idioma:
Zh
Año:
2003
Tipo del documento:
Article