Porcine follistatin cDNA cloning and expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 677-681, 2006.
Article
en Zh
| WPRIM
| ID: wpr-286228
Biblioteca responsable:
WPRO
ABSTRACT
The total RNA was extracted from porcine ovary. Porcine Follistatin cDNA was cloned by RT-PCR. Complete porcine follistatin cDNA coding sequences are presented including 1038 bp of open reading frame. The purified porcine follistatin cDNA was inserted into pGEX-4T-3 vector to construct the prokaryotic fusion protein expression vector. The recombinant expression plasmid was transformed into BL21 (DE3) and expression was induced by IPTG. Protein products were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed that the yield of the Follistatin cDNA was a 63kD protein expression vector. Follistatin protein was expressed in the form of glutathione-S-transferase (GST) fusion protein in E. coli.
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Filogenia
/
Porcinos
/
Proteínas Recombinantes de Fusión
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Química
/
Secuencia de Aminoácidos
/
Clonación Molecular
/
Folistatina
/
Escherichia coli
Límite:
Animals
Idioma:
Zh
Año:
2006
Tipo del documento:
Article