Effects of antenatal administration of dexamethasone and betamethasone on signal transduction of bone morphogenetic protein in the fetal lungs of rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 891-896, 2010.
Article
en Zh
| WPRIM
| ID: wpr-286956
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of antenatal glucocorticoid (dexamethasone and betamethasone) on bone morphogenetic protein (BMP) signal transduction of the rat fetal lungs.</p><p><b>METHODS</b>Fifteen pregnant rats were randomly divided into five groups: the rats treated with dexamethasone for 1 day (1D-DEX) or 3 days (3D-DEX), with betamethasone for 1 day (1D-BEX) or 3 days (3D-BEX) or with normal saline (control group), followed cesarean section on the 19th day of gestation. The mRNA levels of BMP4, BMPR-II, Smad1 and ATF-2 of fetal rat lungs were ascertained by reverse transcriptase polymerase chain reaction (RT-PCR). The expression of BMP4, BMPR-II, Smad1 and ATF-2 antigen expression in fetal lungs was assessed by immune histochemical staining. The expression of BMP4 and BMPR-II was determined by Western blot.</p><p><b>RESULTS</b>The levels of BMP4, BMPR-II and Smad1 mRNA expression were up-regulated in the 1D-BEX, 3D-BEX and 3D-DEX groups compared with those in the control group (P<0.05). The immune histochemiscal analysis showed that the expression of BMP4, BMPR-II, Phospho-Smad1 (pSmad1) and ATF-2 in the 1D-BEX, 3D-BEX and 3D-DEX groups was significantly higher than that in the control group (P<0.01). The results of Western blot demonstrated that the expression of BMP4 and BMPR-II protein increased significantly in the 1D-BEX, 3D-BEX and 3D-DEX groups when compared with the control group (P<0.01).</p><p><b>CONCLUSIONS</b>Betamethasone and dexamethasone may play important roles in the regulation of BMP signal transduction in the rat fetal lungs. Up-regulation of BMP4, BMPR-II and Smad1 might be one of crucial factors for the glucocorticoid-induced maturity of fetal lungs.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Farmacología
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Fisiología
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Betametasona
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Dexametasona
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Transducción de Señal
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Ratas Sprague-Dawley
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Receptores de Proteínas Morfogenéticas Óseas de Tipo II
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Proteína Smad1
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Factor de Transcripción Activador 2
Límite:
Animals
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Pregnancy
Idioma:
Zh
Año:
2010
Tipo del documento:
Article