Gene expression profile in early differentiation of K562 cells induced by matrine / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.</p><p><b>METHODS</b>K562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.</p><p><b>RESULTS</b>From 8465 screened genes, 30 differentially expressed genes were found. Among them 18 showed decreased expression including oncogene and proto-oncogene, signal transduction gene, DNA binding and transcription gene etc, 12 showed increased expression including cell receptor gene, immune-associated gene, metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell.</p><p><b>CONCLUSION</b>The genes, that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.</p>