Expression of Dickkopf-1 in human colon carcinoma cell lines / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of Dickkopf-1(DKK1) in 6 different colon carcinoma cell lines.</p><p><b>METHODS</b>The expression of DKK1 protein were detected in SW480, SW620, HT29, LS174T, LOVO, and HCT116 cells lines using Western blot analysis and immunocytochemistry, and the mRNA levels of DKK1 was detected using quantitative real-time PCR (qPCR).</p><p><b>RESULTS</b>The results of qPCR showed significantly higher expressions of DKK1 mRNA in HT29 and HCT116 cells than in the other 4 cells (P<0.05). Western blotting demonstrated that DKK1 was over-expressed in HCT116 and HT29 cells compared with that in the other cell lines. All the 6 cell lines were found to have positive DKK1 expression by immunocytochemical staining.</p><p><b>CONCLUSION</b>DKK1 shows differential expression patterns among the 6 human colon carcinoma cell lines.</p>