Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 302-306, 2007.
Article
en En
| WPRIM
| ID: wpr-309001
Biblioteca responsable:
WPRO
ABSTRACT
For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H(2)SO(4) and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Espectrometría de Fluorescencia
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Reproducibilidad de los Resultados
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Cromatografía por Intercambio Iónico
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Sensibilidad y Especificidad
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Fluoroquinolonas
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Mezclas Complejas
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Métodos
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Antibacterianos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2007
Tipo del documento:
Article