Toxicity of nano silica particles on rat type I-like alveolar epithelial cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 77-79, 2009.
Article
en Zh
| WPRIM
| ID: wpr-315688
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To access rat lung toxicity of nano silica particles.</p><p><b>METHODS</b>Transmission electron microscope was used to observe size, shape and dispersibility of two silica particles; Size of two particles in water and RPMI 1640 containing 1% FBS were measured using Zeta Potential Analyzer. LDH activities of rat type I-like alveolar epithelial cell line R3/1 cells after 6, 24 and 48 h incubation with 2.5, 5.0, 10.0, 20.0 microg/ml of two silica nano particles were detected by spectrophotometric method; protein carbonylation and MIP-2 release of R3/1 cells after 24 h incubation with 2.5, 5.0, 10.0, 20.0 microg/ml of two silica nano particles were measured using ELISA kits.</p><p><b>RESULTS</b>TEM image showed nano silica particles were round and dispersed evenly; the average sizes of the two silica particles were (43+/-4.2) and (68+/-5.7) nm. Two silica particles had similar size in water and RPMI 1640 containing 1% FBS, respectively. Both nano silica particles in 2.5 approximately 20.0 microg/ml dose range did not cause significant increase of LDH activities (P>0.05), did not elevate protein carbonylation and MIP-2 levels in R3/1 cells (P>0.05).</p><p><b>CONCLUSION</b>Two nano silica particles do not have lung toxicity in 2.5 approximately 20.0 microg/ml dose range.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Células Cultivadas
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Dióxido de Silicio
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Pruebas de Toxicidad
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Toxicidad
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Nanopartículas
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Células Epiteliales Alveolares
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L-Lactato Deshidrogenasa
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Metabolismo
Límite:
Animals
Idioma:
Zh
Año:
2009
Tipo del documento:
Article