Role of connective tissue growth factor (CTGF) in proliferation and migration of pancreatic cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 732-736, 2011.
Article
en Zh
| WPRIM
| ID: wpr-320149
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of connective tissue growth factor (CTGF) in pancreatic cancer and its influence on the proliferation and migration of cancer cells.</p><p><b>METHODS</b>The expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma (50 cases) tissues by immunohistochemistry. The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay, scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.</p><p><b>RESULTS</b>CTGF was overexpressed in both pancreatic cancer cells and tissues. Overxpression of CTGF leads to increased proliferation and migration of PANC-1 cells. The CTGF-transfected PANC-1 cells showed apparent stronger proliferation ability and scratch-repair ability than that of empty vector controls. The results of Boyden chamber test showed that there were 34 cells/field (200× magnificantion) of the CTGF-transfected overexpressing cells, much more than the 11 cells/field of the empty vector control cells; and 6 cells/microscopic field of the Ad5-siCTGF-transfected silenced cells, much less than the 15 cells/field of the control cells.</p><p><b>CONCLUSIONS</b>CTGF is overexpressed in both pancreatic cancer cells in vitro and in vivo, indicating that it may play an important role in the cell proliferation and migration in pancreatic cancer.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Neoplasias Pancreáticas
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Patología
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Proteínas Recombinantes
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Transfección
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Movimiento Celular
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Adenoviridae
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Línea Celular Tumoral
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Proliferación Celular
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Factor de Crecimiento del Tejido Conjuntivo
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Genética
Límite:
Humans
Idioma:
Zh
Año:
2011
Tipo del documento:
Article