Fenofibrate inhibits tumor necrosis factor-alpha-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1383-1387, 2006.
Article
en En
| WPRIM
| ID: wpr-334920
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effects of fenofibrate on TNF-alpha-induced CD40 expression and matrix metalloproteinase (MMP) activity in human vascular endothelial cells (HUVECs).</p><p><b>METHODS</b>Quantitative RT-PCR and flow cytometry were employed to evaluate the effect of fenofibrate on TNF-alpha-induced CD40 mRNA and cell surface CD40 expression in HUVECs, and gelatin zymography was used to determine the effect of fenofibrate on the gelatinolytic activities of MMP-2 and MMP-9 in TNF-alpha-stimulated HUVECs.</p><p><b>RESULTS</b>Fenofibrate at the concentrations of 5x10(-5), 1x10(-4) and 2x10(-4) mol/L significantly reduced TNF-alpha-induced increment of CD40 mRNA and cell surface CD40 expressions (P<0.01), with the maximal inhibition achieved at the concentration of 1x10(-4) mol/L. Fenofibrate at 2x10(-4) mol/L did not further decrease CD40 expression induced by TNF-alpha. Fenofibrate significantly inhibited the stimulatory effect of TNF-alpha on MMP-2 and MMP-9 activities in HUVECs.</p><p><b>CONCLUSION</b>Fenofibrate reduces TNF-alpha-induced increment of CD40 expression and MMP-2 and MMP-9 activities in HUVECs.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Farmacología
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Fenofibrato
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Venas Umbilicales
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ARN Mensajero
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Células Cultivadas
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Factor de Necrosis Tumoral alfa
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Antígenos CD40
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Metaloproteinasa 2 de la Matriz
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Metaloproteinasa 9 de la Matriz
Límite:
Humans
Idioma:
En
Año:
2006
Tipo del documento:
Article