Expression of protein arginine N-methyltransferases in E3 rat models of acute asthma / 南方医科大学学报
Journal of Southern Medical University
; (12): 716-719, 2010.
Article
en Zh
| WPRIM
| ID: wpr-355036
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the expression of protein arginine N-methyltransferase (PRMT) genes in the lung and spleen of E3 rats with acute asthma.</p><p><b>METHODS</b>E3 rats with ovalbumin-induced pulmonary inflammation were divided into two groups (n=10), and the validity of the acute asthma model was evaluated by histological observation with HE and PAS staining and by measurement of NO production. Semi-quantitative RT-PCR was employed to detect the expressions of PRMT1-PRMT6 genes in the lung and spleen tissues of the rats.</p><p><b>RESULTS</b>In the lung tissue of the asthmatic rats, the gene expressions of PRMT1 (P<0.01), PRMT2 (P<0.01), PRMT3 (P<0.05) and PRMT5 (P<0.05) were significantly increased, but the expression of PRMT4 gene (P<0.05) was significantly decreased as compared with those in the control tissue. In the spleen tissue of the asthmatic rats, the expressions of PRMT2 (P<0.05) and PRMT5 genes (P<0.05) showed a significant increase as compared with those in the control rat tissue.</p><p><b>CONCLUSION</b>The gene expressions of PRMTs vary significantly between asthmatic rats and control rats, suggesting that PRMTs play an important role in the post-translational modification process of asthma-related genes.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Proteína-Arginina N-Metiltransferasas
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Ratas Endogámicas
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Asma
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Distribución Aleatoria
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Procesamiento Proteico-Postraduccional
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Enfermedad Aguda
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Clasificación
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Genética
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Metabolismo
Límite:
Animals
Idioma:
Zh
Año:
2010
Tipo del documento:
Article