Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 648-652, 2005.
Article
en Zh
| WPRIM
| ID: wpr-358545
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.</p><p><b>RESULTS</b>Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.</p><p><b>CONCLUSION</b>There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Mapeo Peptídico
/
Región Variable de Inmunoglobulina
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Línea Celular Transformada
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Neoplasias Colorrectales
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Cadenas Pesadas de Inmunoglobulina
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Antígeno B7-1
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Proteoma
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Alergia e Inmunología
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Genética
/
Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Año:
2005
Tipo del documento:
Article