The protective effects of histone deacetylases inhibitor TSA on the mice model of rheumatoid arthritis / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 785-790, 2010.
Article
en Zh
| WPRIM
| ID: wpr-383231
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effects of trichostatin A(TSA)on the mice model of collagen induced arthritis(CIA).Methods Mice model of rheumatoid arthritis(RA)was induced in DBA/1 mice with type Ⅱ collagen.Paws were scored for histological severity of arthritis.The severity of inflammation of mouse joint was evaluated by histological examination.Real-time PGR was used to determine the cytokine mRNA expression.Cytokine production was measured by ELISA from serum,spleen cell culture or dendritic cell and T cell co-culture supematant.T cell proliferation was examined by MTT method.Results TSA can significantly suppress the severity of the arthritis in CIA.IFN-γ was elevated in CIA mice,but was inhibited significantly by TSA introduced either at the same time with immunization or at the onset of manifestation of arthritis.Collagen specific T cell proliferation was significantly suppressed by introduction of TSA.Increased level of IL-4 by T cells was observed in TSA treated group compared to that of control group.Conclusion IL-4 level was increased and played a critical role in the protective effects of TSA in CIA.TSA suppresses the progress of CIA by regulates the balance of Th1/Th2 differentiation.
Texto completo:
1
Banco de datos:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Año:
2010
Tipo del documento:
Article