Analysis of Flavonoids in Semen Hoveniae by HPLC-ESI-MS / 中药新药与临床药理

ABSTRACT

Objective To establish a HPLC-ESI-MS method for simultaneous analysis and determination of flavonoids in Semen Hoveniae. Methods The separation was performed on Acquity UPLCTM BEH C18 column (50 mm× 2. 1 mm,1.7 μm) with a mobile phase of formic acid aqueous solution (B) and MeOH (A). The flow rate was at 1.0 mL/min and detection wavelength was at 290 nm. Samples were analyzed in positive-ion detection model. Results Five kinds of flavonoids in Semen Hoveniae were separated well and were tentatively identified by ESI-MS and by reference to litera-ture data. Conclusion The method is accurate and rapid, and can be used for the identification of flavonoids in Semen Hoveniae.