Study on membrane potential of coronary artery smooth muscle of guinea pig fed with the grains from Keshan disease area / 中国病理生理杂志

ABSTRACT

The reactivity of vascular smooh muscle cell (VSMC) of coronary artery ofguinea pig fed with grains from Keshan disease area were studied by intracellular microelec-trode method. The glass micropipette of tip resistance 30-50 M? filled with 3 M KCl wasinserted from the inner surface of the vasclar segment. The resting membrane potential (Em)of VSMC of coronary artery was observed. The results were as follows: (1) Em ofVSMC of experimental group was -49.80?5.3mV. (2) The Se induced VSMC depolari-zation revealed a dose-response dependent manner in the experimental animal. The degreeof the depolarization was smaller than those in the control guinea pig. (3) KCl and AChcaused membrane depolarization and hyperpolarization respectively in the experimental group,but the changes of Em was higher than those in the control. From these facts it wasconcluded that the reactivity of coronary artery VSMC was increased in experimentalgroup. It was suggested that the vessel's factor play an important role in the pathogenesisof myocardial necrosis in experimental group animal.