Detection of Autoantibodies against Aquaporin-1 in the Sera of Patients with Primary Sjögren's Syndrome
Immune Network
; : 103-109, 2017.
Article
en En
| WPRIM
| ID: wpr-51911
Biblioteca responsable:
WPRO
ABSTRACT
The pathophysiology of glandular dysfunction in Sjögren's syndrome (SS) has not been fully elucidated. Previously, we reported the presence of autoantibodies to AQP-5 in patients with SS, which was associated with a low resting salivary flow. The purpose of this study was to investigate the presence of anti-AQP1 autoantibodies. To detect anti-AQP1 autoantibodies, cell-based indirect immunofluorescence assay was developed using MDCK cells that overexpressed human AQP1. By screening 112 SS and 52 control sera, anti-AQP1 autoantibodies were detected in 27.7% of the SS but in none of the control sera. Interestingly, the sera that were positive for anti-AQP1 autoantibodies also contained anti-AQP5 autoantibodies in the previous study. Different from anti-AQP5 autoantibodies, the presence of anti-AQP1 autoantibodies was not associated with the salivary flow rate. Although anti-AQP1 autoantibodies are not useful as a diagnostic marker, the presence of autoantibodies to AQP1 may be an obstacle to AQP1 gene therapy for SS.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Autoanticuerpos
/
Terapia Genética
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Tamizaje Masivo
/
Técnica del Anticuerpo Fluorescente
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Técnica del Anticuerpo Fluorescente Indirecta
/
Acuaporina 1
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Células de Riñón Canino Madin Darby
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
En
Año:
2017
Tipo del documento:
Article