Improvement on micro-manipulation of fertilized eggs of mice / 第二军医大学学报
Article
en Zh
| WPRIM
| ID: wpr-553158
Biblioteca responsable:
WPRO
ABSTRACT
Objective: To improve the micro-manipulation of mouse fertilized eggs,promoting its survival rate and productivity in pseudo-pregnant mice. Methods: DNA fragments of definite concentration and size were used for micro-manipulation with conventional and improved methods, then their survival rate of manupulated eggs and productivity of pseudo-pregnant mice were analyzed and compared. Results: With improved methods, the survival rate of eggs increased from 60.3% to 79.5%, the productivity of pseudo-pregnant mice and offspring increased from 35.7% to 56.8% and 10.1% to 14.1% respectively. Conclusion: The improved methods of micro-manipulation can be used in transgenic mouse production.
Texto completo:
1
Banco de datos:
WPRIM
Idioma:
Zh
Año:
2001
Tipo del documento:
Article