Assay of Ginsenoside Rg_1 and Rb_1 and Notoginsenoside R_1 in Radix Notoginseng by HPLC/ELSD with Solid Phase Extraction / 中药新药与临床药理

ABSTRACT

Objective To establish a method for determinin g the content of ginsenoside Rg 1 and Rb 1 and notoginsenoside R 1 in Radix Notoginseng.Methods HPLC /ELSD with solid phase extraction(SPE)was applied.The chromatographic conditions were:Hypersil amino -column (200mm ?4.0mm,5?m),acetonitrile -isopropanol -ammon ium acetate(75∶20∶5;acetic acid adjusted pH to5.0)as mobile phrase,flow rate at 0.6mL?min -1 ,column temperature at room temperature,ELSD nebulization at 55℃and flow rate of nitrogen at 2.3L?min -1 Results The linear range of ginseno-side Rg 1 and Rb 1 ,notoginsenoside R 1 was from 1.0to 10.0?g .The average recoveries were 95.5%～102.5%.The inter -day RSD and intra -day were less than 2%and 4%respectively.Conclusion The method is simple and accu-rate,and can be used for the quality contro l of Radix Notoginseng and its preparations.