Isolation of SEN virus and sequence analysis of partial gene in sera from blood donors / 中国输血杂志

ABSTRACT

Objective To identify SEN virus and analyse the sequence of its partial gene in blood donors in China.Methods Two pair primers from ORF1 of SENV genome were designed and samples from blood donors were detected for SENV DNA by nested PCR.The isolates from blood donors were cloned and sequenced.Results 6 isolates were obtained in 329 sera from blood donors.The sequence analysis showed that there were about 52%～100% homology of deduced amino acid between SENV A～H genotypes and 6 isolates from blood donors. The Phylogenetic tree analysis showed that 6 SENV isolates from blood donors belong to SENVH genotype.Conclusion There exists SENVH genotype infection among blood donors in China. SENV may be transmitted through blood transfusion.