Effect of Shh and BM-MSC Synergism on the Proliferation of Hematopoietic Stem Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1523-1530, 2018.
Article
en Zh
| WPRIM
| ID: wpr-689903
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect and mechanism of shh and mesenchymal stem cell(MSC)synergism on the proliferation of hematopoietic stem cells in noninvasive co-culture system in vitro.</p><p><b>METHODS</b>The mesenchymal stem cells were cultured in vitro,CD34 cells were sorted by mini MACS magnetic bead separator,flow cytometry was used to identify the purity of 2 cells. CD34 cells and MSCs were seeded to upper and low of transwell respecibely for non-contact coculture,and add exogenous shh protein for intervenece. The number of MSCs and HSCs,the total amount of RNA,the expression of ki67 and Tie-2 mRNA of HSC,the expression of VEGF and Ang-1 mRNA of MSC were detected for investigating the condition of cell proliferation and the expression of angiogenic factors.</p><p><b>RESULTS</b>The total number of cells,the total amount of RNA and the relative expression of ki67, Tie-2, VEGF and Ang-1 in non-contact co-culture group increased and showed the following trends on the 7th day:the above-mentioned indexes in group MSC + HSC, group shh + HSC were higher than those in group HSC, while those in MSC + shh + HSC Group was higher than those in MSC + HSC and shh + HSC group.</p><p><b>CONCLUSION</b>Angiogenic factors help MSC to proliferate HSC and amplify the CD34 hematopoietic stem/progenitor cells by shh and MSC synergism in vitro coculture system which may be related with angiogenic factors.</p>
Texto completo:
1
Banco de datos:
WPRIM
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Año:
2018
Tipo del documento:
Article