Evaluation of two different enzyme-linked immunosorbent assay for severe fever with thrombocytopenia syndrome virus diagnosis
Clinical and Experimental Vaccine Research
; : 82-86, 2018.
Article
en En
| WPRIM
| ID: wpr-739633
Biblioteca responsable:
WPRO
ABSTRACT
To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strains compared with those of Gn-based ELISA tests. However, Gn-based ELISA showed more genotype specificity and specificity. These result suggested that NP-based ELISA test could be applicable for general sero-prevalence studies of SFTSV infections, while Gn-based ELISA could be applicable for a certain specific genotype sero-prevalence study.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Trombocitopenia
/
Ensayo de Inmunoadsorción Enzimática
/
Sensibilidad y Especificidad
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Proteínas de la Nucleocápside
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Diagnóstico
/
Fiebre
/
Genotipo
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2018
Tipo del documento:
Article