Biocompatibility and Immunotoxicology of the Preclinical Implantation of a Collagen-based Artificial Dermal Regeneration Matrix / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 829-842, 2018.
Article
en En
| WPRIM
| ID: wpr-772241
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#Graft rejection, with the possibility of a violent immune response, may be severe and life threatening. Our aim was to thoroughly investigate the biocompatibility and immunotoxicology of collagen-based dermal matrix (DM) before assessment in clinical trials.@*METHODS@#DM was subcutaneously implanted in BALB/c mice in two doses to induce a potential immune response. The spleen and lymph nodes were assessed for shape, cell number, cell phenotype via flow cytometry, cell activation via CCK8 kit, Annexin V kit, and Ki67 immunostaining. Serum samples were used to measure antibody concentration by enzyme-linked immunosorbent assay. Local inflammation was analyzed by histology and immunohistochemistry staining. Data analysis was performed by one-way ANOVA and non-parametric tests.@*RESULTS@#Our data illustrate that the spleen and lymph node sizes were similar between the negative control mice and mice implanted with DM. However, in the high-dose DM (DM-H) group, the total cell populations in the spleen and lymph nodes, T cells and B cells in the spleen had slight increases in prophase, and the low-dose DM (DM-L) group did not display gross abnormities. Moreover, DM-H initiated moderate cell activation and proliferation in the early phase post-immunization, whereas DM-L did not. Neither DM-H nor DM-L implantation noticeably increased IgM and IgG serum concentrations. Examination of the local cellular response revealed only benign cell infiltration and TNF-α expression in slides of DM in the early phase.@*CONCLUSION@#Overall, DM-H may have induced a benign temporary acute immune response post-implantation, whereas DM-L had quite low immunogenicity. Thus, this DM can be regarded as a safe product.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Prótesis e Implantes
/
Bazo
/
Cirugía General
/
Materiales Biocompatibles
/
Colágeno
/
Dermis
/
Alergia e Inmunología
/
Citometría de Flujo
/
Inmunidad Celular
/
Ganglios Linfáticos
Límite:
Animals
Idioma:
En
Año:
2018
Tipo del documento:
Article