Identification of metabolites of Danshensu in rats / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3933-3939, 2018.
Article
en Zh
| WPRIM
| ID: wpr-775394
Biblioteca responsable:
WPRO
ABSTRACT
To identify the metabolites of Danshensu in plasma and urine in rats by using UHPLC-LTQ-Orbitrap method. After oral gavage of Danshensu CMC-Na suspension in SD rats, urine and plasma samples were collected and processed by solid phase extraction. ACQUITY UPLC BEH C₁₈ column (2.1 mm×100 mm, 1.7 μm) was utilized, with 0.1% formic acid (A)-acetonitrile (B) solution as the mobile phase for gradient elution. Negative electrospray ion mode based data-acquisition method was established to collect the mass spectrometry data of biological samples. As a result, Danshensu and 21 Danshensu Ⅰ phase and Ⅱ phase metabolites were finally identified according to the accurate mass measurements, mass fragmentation behaviors and comparing with the reference standards. The main metabolic pathways included dehydration, methylation, glucuronide conjugation, sulfate conjugation and their composite reactions. Consequently, our study expounded metabolites of Danshensu in rats based on UHPLC-LTQ-Orbitrap method and provided a reference for further researches on therapeutic material basis and mechanism of Danshensu.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Espectrometría de Masas
/
Orina
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Sangre
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Cromatografía Líquida de Alta Presión
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Ratas Sprague-Dawley
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Lactatos
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Metabolismo
Tipo de estudio:
Diagnostic_studies
/
Guideline
Límite:
Animals
Idioma:
Zh
Año:
2018
Tipo del documento:
Article