Screening for proteins interacting with ataxin-3, the gene product of SCA3/MJD / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 40-44, 2006.
Article
en Zh
| WPRIM
| ID: wpr-813770
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To screen for proteins interacting with ataxin-3 by yeast two-hybrid system 3, and to discuss the function of ataxin-3 and pathogenesis of spinocerebellar ataxia type 3 and Machado-Joseph disease (SCA3/MJD).@*METHODS@#First we sub-cloned the full reading frame of both wild-type and mutant ataxin-3 into carrier pGBKT7 (ataxin-3-bait), and then screened human brain cDNA library with ataxin-3-bait.@*RESULTS@#We found five positive clones in 6.5 x 10(6) transformers. After sequencing, we knew all of them were novel ataxin-3 interacting proteins. Three were corresponded to the known sequences coding the known proteins, which were human Rho GDP dissociation inhibitor alpha, small ubiquitin-like modifier 1, and human neuronal amiloride-sensitive cation channel 2. Another two of the five were unknown.@*CONCLUSION@#Small ubiquitin-like modifier 1 probably interacted with ataxin-3, suggesting that the sumoylation probably participated in post-translation modifying of ataxin-3 and pathogenesis of SCA3/MJD.
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Proteínas Represoras
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Levaduras
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Encéfalo
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Proteínas Nucleares
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Degeneraciones Espinocerebelosas
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Biblioteca de Genes
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Técnicas del Sistema de Dos Híbridos
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Mapeo de Interacción de Proteínas
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Ataxina-3
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Genética
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Año:
2006
Tipo del documento:
Article