Construction of fluorescent transgenic zebrafish Tg (ttn.2: EGFP) / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1804-1814, 2023.
Article
en Zh
| WPRIM
| ID: wpr-981171
Biblioteca responsable:
WPRO
ABSTRACT
In order to develop a transgenic zebrafish line with green fluorescent protein (enhanced green fluorescent protein, EGFP) expressed specifically in muscle and heart, the recombinant expression vector constructed using the zebrafish ttn.2 gene promoter fragment and EGFP gene coding sequence and the capped mRNA of Tol2 transposase were co-injected into the zebrafish 1-cell stage embryos. The stable genetic Tg (ttn.2 EGFP) transgenic zebrafish line was successfully developed by fluorescence detection, followed by genetic hybridization screening and molecular identification. Fluorescence signals and whole-mount in situ hybridization showed that EGFP expression was located in muscle and heart, the specificity of which was consistent with the expression of ttn.2 mRNA. Inverse PCR showed that EGFP was integrated into chromosomes 4 and 11 of zebrafish in No. 33 transgenic line, while integrated into chromosome 1 in No. 34 transgenic line. The successful construction of this fluorescent transgenic zebrafish line, Tg (ttn.2 EGFP), laid a foundation for the research of muscle and heart development and related diseases. In addition, the transgenic zebrafish lines with strong green fluorescence can also be used as a new ornamental fish.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Pez Cebra
/
Animales Modificados Genéticamente
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Regiones Promotoras Genéticas
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Proteínas de Pez Cebra
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Proteínas Fluorescentes Verdes
Límite:
Animals
Idioma:
Zh
Año:
2023
Tipo del documento:
Article