Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats
J. appl. oral sci
; 28: e20190140, 2020. tab, graf
Article
em En
| LILACS, BBO
| ID: biblio-1056590
Biblioteca responsável:
BR28.1
ABSTRACT
Abstract Objective:
The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model.Methodology:
Thirty male Wistar rats were divided into three groups 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed.Results:
ABL was significantly lower in Group SO compared to Group C (p 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p 0.0033, p 0.0075, respectively).Conclusion:
Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group.Palavras-chave
Texto completo:
1
Base de dados:
BBO
/
LILACS
Assunto principal:
Ozônio
/
Periodontite
/
Perda do Osso Alveolar
/
Subunidade alfa do Fator 1 Induzível por Hipóxia
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2020
Tipo de documento:
Article