WITHDRAWN: Selection of reference genes in five types of human tissuesfor normalization of gene expression studies in infectious diseases
Meira-Strejevitch, Cristina Silva; Pereira-Chioccola, Vera Lucia; Maia, Marta Marques; Hipólito, Daise Damaris Carnietto de; Wang, Hui-Tzu Lin; Motoie, Gabriela; Gomes, Aparecida Helena de Souza; Kanamura, Cristina Takami; Martines, Roosecelis Brasil; Mattos, Cinara Cássia Brandão de; Frederico, Fábio Batista; Mattos, Luiz Carlos de; Mattos, Cinara Cássia Brandão de; Frederico, Fábio Batista; Siqueira, Rubens Camargo; Previato, Mariana; Barbosa, Amanda Pires; Murata, Fernando Henrique Antunes.
Gene
; 12(16): 30816-30822, 2016.
Artigo
Inglês
| SES-SP, SES SP - Instituto Dante Pazzanese de Cardiologia, SES-SP | ID: biblio-1063066
Gene expression analyses based on messenger RNA (mRNA) expression require accurate datanormalization. When using endogenous reference genes, these have to be carefully validated. Validated reference genes vary greatly depending on tissue, cell subsets and experimental context.The aim of this study was to identify reference genes that present more stable mRNA levels amongindividuals in peripheral blood mononuclear cells (PBMC); fresh skin biopsies; lung and brainautopsies as well as, skin biopsies formalin-fixed paraffin-embedded (FFPE). Therefore, 6endogenous reference genes were evaluated by quantitative real-time polymerase chain reaction 18SrRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), TATA box-binding protein (TBP),beta-2-microbolin (B2M), ubiquitin C (UBC) and mitochondrially encoded ATP synthase 6 (MTATP6).Furthermore, validation of their stability and suitability as reference genes was determined bythe geNorm program. The results show that in PBMC and fresh skin biopsies, TBP and UBC wereidentified as the most stable, while in FFPE lung autopsies and skin biopsies, GAPDH and B2M, andin FFPE brain autopsies, GAPDH and UBC turned out to be the most stable...
Biblioteca responsável:
BR79.1
Localização: BR79.1
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