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Fungi in dialysis water and dialysate: occurrence, susceptibility to antifungal agents and biofilm production capacity
Anversa, Laís; Lara, Bruna Rossini; Romani, Caroline Demai; Saeki, Erika Kushilawa; Nascentes, Gabriel Antonio Nogueira; Bonfietti, Lucas Xavier; Melhem, Márcia de Souza Carvalho; Ruiz, Luciana da Silva; Camargo, Carlos Henrique; Pereira, Virgínia Bodelão Richini.
Afiliação
  • Anversa, Laís; Instituto Adolfo Lutz. BR
  • Lara, Bruna Rossini; Instituto Adolfo Lutz. BR
  • Romani, Caroline Demai; Instituto Adolfo Lutz. BR
  • Saeki, Erika Kushilawa; Instituto Adolfo Lutz. BR
  • Nascentes, Gabriel Antonio Nogueira; Instituto Federal do Triângulo Mineiro. BR
  • Bonfietti, Lucas Xavier; Instituto Adolfo Lutz. BR
  • Melhem, Márcia de Souza Carvalho; Instituto Adolfo Lutz. BR
  • Ruiz, Luciana da Silva; Instituto Adolfo Lutz. BR
  • Camargo, Carlos Henrique; Instituto Adolfo Lutz. BR
  • Pereira, Virgínia Bodelão Richini; Instituto Adolfo Lutz. BR
Journal of water & health ; 00(0): 1-12, 2021. tab, graf
Article em En | CONASS, SES-SP, SESSP-IALPROD, SES-SP | ID: biblio-1292361
Biblioteca responsável: BR91.2
Localização: BR76.1; Digital
ABSTRACT
The aim of this study was to investigate the occurrence of fungi in dialysis water and dialysate, in addition to evaluating the susceptibility to antifungals and the biofilm production capacity of isolated microorganisms. The samples were collected in three hemodialysis units in Bauru (Brazil), every 15 days (July 2017­June 2018) at post-reverse osmosis, reuse, and dialysate points. The fungi were isolated by spread plate on Sabouraud dextrose agar. Filamentous fungi were phenotypically identified and yeasts were subjected to molecular evaluation of the ITS region. Susceptibility test to antifungals was carried out by the broth microdilution method and biofilm production capacity was evaluated in microtiter plates using crystal violet staining. Fungi were isolated in 52/216 (24.1%) samples, with an average count of 16.3 (10­40) CFU/ mL. Overall, 61 microorganisms were identified, with 54 (88.5%) filamentous fungi and 7 (11.5%) yeasts. The main genera included were Penicillium, Cladosporium, Scedosporium, Rhinocladiella, Fusarium, and Emmonsia. Most isolates showed high values of minimum inhibitory concentration for 5-flucytosine and fluconazole and 35/45 (77.8%) isolates were classified as strong producers of biofilm. In order to increase the safety of the dialysis process, the adoption of control measures and monitoring of fungi in hemodialysis fluids is suggested.
Assuntos

Texto completo: 1 Coleção SES: Producao_cientifica Base de dados: CONASS / SES-SP / SESSP-IALPROD Assunto principal: Chrysosporium / Soluções para Diálise / Diálise / Antifúngicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleção SES: Producao_cientifica Base de dados: CONASS / SES-SP / SESSP-IALPROD Assunto principal: Chrysosporium / Soluções para Diálise / Diálise / Antifúngicos Idioma: En Ano de publicação: 2021 Tipo de documento: Article