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Chemical profile, anti 5-lipoxygenase and cyclooxegenase inhibitory effects of ginger (Zingiber officinale) rhizome, callus and callus treated with elicitors / Perfil químico, anti-5-lipoxigenase e efeitos inibitórios da ciclooxegenase do rizoma de gengibre (Zingiber officinale), do calo e calos tratados com elicitores
Ali, Ammar Mohammed Ahmed; El-Nour, Mawahib ElAmin Mohamed; Al-Atar, Abdulrahman Abdullah; Mohammad, Owais; El-Sheikh, Mohamed Abdel-Raouf; Qahtan, Ahmed Ali; Abdel-Salam, Eslam Mohamed; Yagi, Sakina Mohamed.
Afiliação
  • Ali, Ammar Mohammed Ahmed; Hajjah University. Faculty of Education. Department of Biology. Hajjah. YE
  • El-Nour, Mawahib ElAmin Mohamed; AL Neelain University. Faculty of Science and Technology. Department of Biology and Biotechnology. Khartoum. SD
  • Al-Atar, Abdulrahman Abdullah; King Saud University. College of Science. Department of Botany and Microbiology. Riyadh. SA
  • Mohammad, Owais; Aligarh Muslim University. Biotechnology Unit. Molecular Immunology Lab1. Aligarh. IN
  • El-Sheikh, Mohamed Abdel-Raouf; King Saud University. College of Science. Department of Botany and Microbiology. Riyadh. SA
  • Qahtan, Ahmed Ali; King Saud University. College of Science. Department of Botany and Microbiology. Riyadh. SA
  • Abdel-Salam, Eslam Mohamed; King Saud University. College of Science. Department of Botany and Microbiology. Riyadh. SA
  • Yagi, Sakina Mohamed; University of Khartoum. Faculty of Science. Department of Botany. Khartoum. SD
Ciênc. rural (Online) ; 52(10): e20210372, 2022. tab, graf
Article em En | LILACS, VETINDEX | ID: biblio-1364722
Biblioteca responsável: BR68.1
ABSTRACT
The present study investigated the chemical profiles and evaluated the inhibitory effect against 5-Lipoxygenase (5-Lox) activity for extracts of ginger rhizome, callus, and callus treated with the elicitors; yeast extract (100, 300 and 500 mg/L), glycine (100, 200 and 300 mg/L) and salicylic acid (100 and 200 mg/L). Oils and chloroform methanol (CM) extracts were prepared by maceration in petroleum ether and CM (11, v/v), respectively. Chemical profiles were determined by gas chromatography/mass spectrometry (GC/MS) analysis. Oil of the callus recorded higher 5-Lox inhibitory effect (IC50 58.33±4.66 µg/mL) than the oil of rhizome (IC50168.34±15.64 µg/mL) and comparable to that of the positive control; Nordihydroguaiaretic acid (IC50 61.25±1.02 µg/mL). The chemical profile of the callus oil contained large amounts of fatty acids, mainly the unsaturated fatty acid oleic acid (31.11%) and saturated fatty acid palmitic acid (28.56%). Elicitors modified the chemical profile of the callus and ameliorated the anti-5-Lox activity of CM extract of the callus. CM extracts of callus treated with 100 and 300 mg/L yeast extract and 50 mg/L salicylic acid significantly suppressed (P ≤ 0.05) the 5-Lox activity by 33.16%, 25.46% and 16%, respectively as compared to the CM extract of untreated callus. In conclusion, ginger callus could be considered as a valuable dietary supplement in the treatment of various inflammatory disorders.
RESUMO
O presente estudo teve como objetivo investigar os perfis químicos e avaliar o efeito inibitório da atividade da 5-Lipoxigenase (5-Lox) em extratos de rizoma, calo e calo de gengibre tratados com os eliciadores; extrato de levedura (100, 300 e 500 mg / L), glicina (100, 200 e 300 mg / L) e ácido salicílico (100 e 200 mg / L). Extratos de óleos e clorofórmio metanol (CM) foram preparados por maceração em éter e CM (1 1, v / v), respectivamente. Os perfis químicos foram determinados por análise de cromatografia gasosa / espectrometria de massa (GC / MS). O óleo do calo registrou maior efeito inibitório de 5-Lox (IC50 58,33 ± 4,66 µg / mL) do que o óleo de rizoma (IC50168,34 ± 15,64 µg / mL) e comparável ao do controle positivo; Ácido nordi-hidroguaiarético (IC50 61,25 ± 1,02 µg / mL). O perfil químico do óleo de calo continha grandes quantidades de ácidos graxos, principalmente o ácido graxo insaturado ácido oleico (31,11%) e ácido graxo saturado palmítico (28,56%). Os elicitores modificaram o perfil químico do calo e melhoraram a atividade anti-5-Lox do extrato de CM do calo. Extratos de CM de calos tratados com 100 e 300 mg / L de extrato de levedura e 50 mg / L de ácido salicílico suprimiram significativamente (P ≤ 0,05) a atividade de 5-Lox em 33,16%, 25,46% e 16%, respectivamente, em comparação com o extrato de CM de calo não tratado. Em conclusão, o calo de gengibre pode ser considerado um suplemento dietético valioso no tratamento de vários distúrbios inflamatórios.
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Texto completo: 1 Base de dados: LILACS / VETINDEX Assunto principal: Lipoxigenase / Ácido Salicílico / Zingiber officinale / Rizoma Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: LILACS / VETINDEX Assunto principal: Lipoxigenase / Ácido Salicílico / Zingiber officinale / Rizoma Idioma: En Ano de publicação: 2022 Tipo de documento: Article