The type III secretion system (T3SS)-Translocon of atypical enteropathogenic Escherichia coli (aEPEC) can mediate adherence
Santos, Fernanda F; Abe, Cecília Mari; Piazza, Roxane Maria Fontes; Elias, Waldir Pereira; Bryant, Jack A; Hernandes, Rodrigo T; Kitamura, Felipe C; Castro, Felipe S; Valiatti, Tiago B; Henderson, Ian R; Gomes, Tânia A. T; Yamamoto, Denise.
Front Microbiol
; 10: 1527, 2019.
Artigo
Inglês
| SES-SP, SES SP - Instituto Butantan, SES-SP | ID: but-ib16112
The intimin protein is the major adhesin involved in the intimate adherence of atypicalenteropathogenicEscherichia coli(aEPEC) strains to epithelial cells, but little is knownabout the structures involved in their early colonization process. A previous studydemonstrated that the type III secretion system (T3SS) plays an additional role in theadherence of anEscherichia albertiistrain. Therefore, we assumed that the T3SS couldbe related to the adherence efficiency of aEPEC during the first stages of contactwith epithelial cells. To test this hypothesis, we examined the adherence of sevenaEPEC strains and theireae(intimin) isogenic mutants in the standard HeLa adherenceassay and observed that all wild-type strains were adherent while five isogeniceaemutants were not. The twoeaemutant strains that remained adherent were then usedto generate theeae/escNdouble mutants (encoding intimin and the T3SS ATPase,respectively) and after the adherence assay, we observed that one strain lost itsadherence capacity. This suggested a role for the T3SS in the initial adherence stepsof this strain. In addition, we demonstrated that this strain expressed the T3SS atsignificantly higher levels when compared to the other wild-type strains and that itproduced longer translocon-filaments. Our findings reveal that the T3SS-transloconcan play an additional role as an adhesin at the beginning of the colonization processof aEPEC.
Biblioteca responsável:
BR78.1
Localização: BR78.1
Texto completo
Documentos relacionados
