Tumour and serum MGMT promoter methylation and protein expression in glioblastoma patients
Clin. transl. oncol. (Print)
; 13(9): 677-685, sept. 2011. tab, ilus
Article
em En
| IBECS
| ID: ibc-125873
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
INTRODUCTION: Methylation of the promoter of the MGMT gene and MGMT protein expression are recognized as predictive markers for response to alkylating chemotherapy in glioblastoma (GB). MATERIAL AND METHODS: We have assessed MGMT methylation with the methylation-specific polymerase chain reaction (MSP) in tumor samples from 70 GB patients and in serum samples from 37 of these patients. We have also assessed MGMT protein expression by immunohistochemical (IHC) analysis in tissue samples from 63 of these patients. RESULTS: We found concordance between MGMT methylation status in tissue and serum (Cohen's Kappa = 0.586; p<0.0001). MSP for detection of non-methylated MGMT promoter in serum showed a sensitivity of 95.4% and a specificity of 60%, while the IHC methylation test showed a low specificity (8.9%). Patients whose MGMT promoter was methylated in tissue attained longer progression-free and overall survival. In the multivariate analysis, serum MGMT promoter methylation emerged as an independent factor for longer progression-free and overall survival. CONCLUSION: Serum-based MGMT methylation analysis offers a promising alternative to tumor-based MGMT analysis in cases where tissue samples are unavailable (AU)
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Base de dados:
IBECS
Assunto principal:
Neoplasias Encefálicas
/
Metilases de Modificação do DNA
/
Biomarcadores Tumorais
/
Glioblastoma
/
Proteínas Supressoras de Tumor
/
Enzimas Reparadoras do DNA
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Aged
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article