Detection of human papillomavirus DNA by the hybrid capture assay
Braz. j. infect. dis
; 7(2): 121-125, Apr. 2003. tab
Article
em En
| LILACS
| ID: lil-351155
Biblioteca responsável:
BR1.1
ABSTRACT
Human Papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide. Consequently, it would be useful to evaluate HPV testing to screen for cervical cancer. Recently developed, the second-generation Hybrid Capture (HCA II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 18 HPV types, divided into high and low-risk groups. We evaluated 1055 women for HPV infection with the HCA II test. Five hundred and ten (48.3 percent) of these women had HPV infection; 60 (11.8 percent) had low cancer-risk HPV DNA; 269 (52.7 percent) had high-risk HPV types and 181 (35.5 percent) had both groups. Hence, 450 women (88.2 percent) in this HPV-infected group had at least one high risk HPV type, and were therefore considered to be at high risk for cancer. Among the group with Papanicolaou (Pap) test results, the overall prevalence of HPV DNA was 58.4 percent. Significant differences in HPV infection of the cervix were detected between Pap I (normal smears) and Pap IV (carcinomas) (p<0.0001). Values of HPV viral load obtained for Pap I and SILs were significantly different, with an upward trend (p<0.0001), suggesting a positive correlation between high viral load values and risk of SIL. Because of the high costs of the HCA II test, its use for routine cervical mass screening cannot be recommended in poor countries. Nevertheless, it is a useful tool when combined with cytology, diagnosing high-risk infections in apparently normal tissues. Use of this technique could help reduce the risk of cancer
Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Papillomaviridae
/
DNA Viral
/
Displasia do Colo do Útero
/
Neoplasias do Colo do Útero
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Infecções por Papillomavirus
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
/
Etiology_studies
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Prevalence_studies
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Risk_factors_studies
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Screening_studies
Limite:
Adult
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Female
/
Humans
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article