Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection
Mem. Inst. Oswaldo Cruz
; 103(2): 195-200, Mar. 2008. graf, tab
Article
em En
| LILACS
| ID: lil-480634
Biblioteca responsável:
BR1.1
ABSTRACT
Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23 percent, and only AdV C was detected.
Palavras-chave
Texto completo:
1
Base de dados:
LILACS
Assunto principal:
Infecções Respiratórias
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Infecções por Adenovirus Humanos
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Adenovírus Humanos
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Child
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Female
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Humans
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Male
País como assunto:
Mexico
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article
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Project document