Synthesis and cytotoxicity evaluation of granular magnesium substituted beta-tricalcium phosphate
J. appl. oral sci
; 21(1): 37-42, 2013. ilus, graf
Article
em En
| LILACS, BBO
| ID: lil-684993
Biblioteca responsável:
BR28.1
ABSTRACT
Objective:
The aim of this study was to produce dense granules of tricalcium phosphate (β-TCP) and magnesium (Mg) substituted β-TCP, also known as β-TCMP (Mg/Ca=0.15 mol), in order to evaluate the impact of Mg incorporation on the physicochemical parameters and in vitro biocompatibility of this novel material. Material andMethods:
The materials were characterized using X-ray diffraction (XRD), infrared spectroscopy (FTIR), electron microscopy and inductively coupled plasma (ICP). Biocompatibility was assayed according to ISO 10993-122007 and 74052008, by two different tests of cell survival and integrity (XTT and CVDE).Results:
The XRD profile presented the main peaks of β-TCP (JCPDS 090169) and β-TCMP (JCPDS 130404). The characteristic absorption bands of TCP were also identified by FTIR. The ICP results of β-TCMP granules extract showed a precipitation of calcium and release of Mg into the culture medium. Regarding the cytotoxicity assays, β-TCMP dense granules did not significantly affect the mitochondrial activity and relative cell density in relation to β-TCP dense granules, despite the release of Mg from granules into the cell culture medium.Conclusion:
β-TCMP granules were successfully produced and were able to release Mg into media without cytotoxicity, indicating the suitability of this promising material for further biological studies on its adequacy for bone therapy.Palavras-chave
Texto completo:
1
Base de dados:
BBO
/
LILACS
Assunto principal:
Materiais Biocompatíveis
/
Fosfatos de Cálcio
/
Magnésio
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article