Single-step method for the determination of the amount of the common deletion in mitochondrial DNA.
Biotechniques
; 26(2): 290-2, 294, 296 passim, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-10023541
ABSTRACT
A fluorescent-based quantitative PCR assay to measure the percentage of the common deletion in rat mitochondria is reported. The amount of the common deletion is measured relative to the amount of total mitochondrial (mt)DNA. The use of a heterologous competitor construct allows the reaction to be monitored to ensure that exponential accumulation of products occurs. The use of fluorescence-labeled primers provides a safe and sensitive means to quantify products without any adjustment for size. This assay will allow the quantitative determination of the common deletion with one dilution range.
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Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Análise Mutacional de DNA
/
Reação em Cadeia da Polimerase
/
Deleção de Genes
Limite:
Animals
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article