[Effect of pH on cea gene expression in cells irradiated with UV-light and unirradiated Escherichia coli cells]. / Vliianie pH na ékspressiiu gena cea u obluchennykh UF-svetom i neobluchennykh kletok Escherichia coli.
Genetika
; 34(11): 1480-3, 1998 Nov.
Article
em Ru
| MEDLINE
| ID: mdl-10096025
ABSTRACT
The effect of pH on the expression of the cea gene encoding colicin EI in Escherichia coli was investigated by measuring the beta-galactosidase activity in the UV-irradiated growing cells carrying the cea-lacZ fusion. Maximum activity was observed at pH 7, and inhibition of expression was observed at pH 6 and pH 8. Treatment of the irradiated cells with 50-mM acetate increased inhibition at pH 6.0-7.5. No correlation between cea expression and the rate of cell growth was observed at different pH levels. Preliminary treatment with acetate at pH 7 reduced the expression of the recA gene, which participates in the regulation of the cea gene to 33% in irradiated cells and to 25% in nonirradiated cells.
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Colicinas
/
Escherichia coli
/
Concentração de Íons de Hidrogênio
Idioma:
Ru
Ano de publicação:
1998
Tipo de documento:
Article