Mutation S543N in the thumb subdomain of the Taq DNA polymerase large fragment suppresses pausing associated with the template structure.
FEBS Lett
; 448(1): 145-8, 1999 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-10217428
ABSTRACT
Substitution of Asn for the conserved Ser543 in the thumb subdomain of the Taq DNA polymerase large fragment (Klentaq DNA polymerase) prevents pausing during DNA synthesis and allows the enzyme to circumvent template regions with a complex structure. The mutant enzyme (KlentaqN DNA polymerase) provides specific PCR amplification and sequencing of difficult templates, e.g. those with a high GC% content or strong secondary structure.
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Base de dados:
MEDLINE
Assunto principal:
Asparagina
/
Serina
/
Mutação Puntual
/
Taq Polimerase
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article