Rapid spine delivery and redistribution of AMPA receptors after synaptic NMDA receptor activation.
Science
; 284(5421): 1811-6, 1999 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-10364548
ABSTRACT
To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.
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Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Receptores de N-Metil-D-Aspartato
/
Receptores de AMPA
/
Dendritos
/
Plasticidade Neuronal
/
Neurônios
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article